54
ICCS 2011
P18
FLOW CYTOMETRIC ANALYSIS OF LYMPHOMA
CELL SIZE OF DIFFUSE LARGE B-CELL
LYMPHOMA
Michelle Huang
1
, Farzaneh Sayedian
1
, Aaron Cotrell
1
,
Thomas Fennell
1
, Alaa Muslimani
1
, Xia Chen
2
, Smith
Marc
1
, James Huang
1
1
William Beaumont School of Medicine, William
Beaumont Hospital , Royal Oak, MI, USA,
2
William
Beaumont School of Medicine, William Beaumont
Hospital , Troy, MI, USA
INTRODUCTION:
Diffuse large B-cell lymphoma
(DLBCL) is a tumor of diffuse growth pattern of
large B-cells. The size of lymphoma cells is typically
evaluated based on microscopic comparison between
neoplastic B-cells and normal macrophage or normal
lymphocyte on a hematoxylin and eosin stained
section. It is not clear if the size of lymphoma cells
in DLBCL can be objectively measured by fow
cytometry based on forward scatter (FSC). This
study seeks to determine if FSC is different between
DLBCL and follicular lymphoma grade 3 (FL3) and
if it is potentially helpful in lymphoma classifcation.
MATERIALS AND METHODS:
We retrospectively
analyzed the fow cytometric listmode data of 26
cases of DLBCL and 26 cases of FL3. The mean
channels of FSC of intact neoplastic B-cells and
internal reactive T-cells were collected and compared
with Student’s t-test.
RESULTS:
The mean FSC of
internal reactive T-cells within the lymphoma tissue
was about the same (425 vs. 420, p=0.145) between
DLBCL and FL3. The mean FSC of neoplastic
B-cells of DLBCL was signifcantly higher than that
of FL3 (589 vs. 491, p<0.001). The difference of
FSC between lymphoma cells of DLBCL and internal
reactive T-cells was 164 (95% confdence limits: 125
and 203). The difference of FSC between lymphoma
cells of FL3 and T-cells was 72 (95% confdence
limits: 40 and 104).
CONCLUSION:
Our fndings
show that FSC of neoplastic B-cells is signifcantly
different between DLBCL and FL3. Such difference
can be objectively measured using internal reactive
T-cells as a reference and applied in classifcation of
B-lymphoma.
POSTER ABSTRACTS