61
OCTOBER 16-18, 2011 • PORTLAND, OR
P25
Standardization of Multiple Cytometers using
BD™ CS&T Beads for Consistent Results
Ellen M. Meinelt, Mervi Reunanen, Maria C. Jaimes,
Mark Edinger
BD Biosciences, San Jose, CA, USA
Fluorescent intensity of populations is monitored in
immunophenotypic assays. Changes in the intensity
may be due to treatment the patient is receiving
or variation in instrument settings. In order to
generate high quality, reproducible data and maintain
consistency or time or across multiple cytometers,
a process has been created. This process includes
using the BD FACSDiva
™
software which contains
the Cytometer Setup & Tracking (CS&T) module
for: •Defning a Cytometer Baseline •Establishing
2.5 times the SD of the Electronic Noise for each
fuorescent parameter •Creating Application Settings
•Verifying the voltages with positively stained cells
•Creating Target values using the BD™ CS&T beads
and •Transferring the cytometer confguration,
experiment, and bright bead target values to other
cytometers Digital fow cytometers with the same
lasers and optical confgurations can be standardized
with the process presented here. Flow cytometers
with different lasers and optical confgurations can
also be standardized with slight modifcations to
the process. Data will be shown to demonstrate the
outcome of this process using stained samples.
POSTER ABSTRACTS