63
OCTOBER 16-18, 2011 • PORTLAND, OR
P27
Analysis of the Immunophenotypic Aberrancies in
the Maturing Myeloid and Monocytic Compartment
in Acute Myeloid Leukemia by Flow Cytometry
Ying Pei, Jason Schallheim, Guang Fan
Oregon Health Science University, Portland, OR, USA
Dysplastic morphologic features are commonly
present in the maturing granulocytes and monocytes
in acute myeloid leukemia (AML). So far, no study has
been reported to investigate the immunophenotypic
characters in these maturing compartments in
AML comparing to the normal individuals by our
knowledge. The purpose of this study is to investigate
the immunophenotypic aberrancies in the maturing
myeloid and monocytic compartments in AML. 20
cases of AML diagnosed in OHSU between 2008 and
2011 were reviewed. The fow cytometry results of
peripheral blood and bone marrow aspiration were
analyzed. Flow cytometry antibody panels included
CD71/CD117/CD33/CD13/CD34/HLA-DR/CD16/
CD45; CD15/CD64/CD123/CD56/CD34/CD14/CD11b/
CD45; CD5/CD10/CD34/CD20/CD19/CD45; CD2/
CD7/CD4/CD56/CD5/CD8/CD3/CD45. Overall 85% of
the cases showed immunophenotypic aberrancies in
the maturing myeloid and monocytic compartments. In
the myeloid compartment, the most common fnding
was decreased or lack of expression of CD10 (50%),
which was followed by decreased expression of
CD16 (20%) and aberrant expression of CD56 (15%),
CD123 (15%) and HLA-DR (10%). In the maturing
monocytic compartment, abnormal immunophenotypic
fndings included aberrant expression of CD 123
(25%) and CD 56 (20%) and decreased expression
of CD14 (20%). In conclusion, immunophenotypic
aberrancies are commonly present in the maturing
myeloid and monocytic compartments in AML. Most
of these abnormal immunophenotypic features have
also been reported in the cases of myelodysplastic
syndrome, which suggests that maturing
compartments in most of AML has immunologic
features of dysplastic maturation. Future studies
will expand the case numbers and focus on the
correlation of immunophenotypic aberrancies with the
molecular studies and explore prognostic implications.
POSTER ABSTRACTS